Jun Chen

Positions:

Professor of Medicine

Medicine, Hematologic Malignancies and Cellular Therapy
School of Medicine

Member of the Duke Cancer Institute

Duke Cancer Institute
School of Medicine

Affiliate of the Duke Regeneration Center

Regeneration Next Initiative
School of Medicine

Education:

M.D. 1991

Sun Yat Sen University (China)

Postdoctoral Fellow

Stanford University, School of Medicine

Publications:

BAFF promotes heightened BCR responsiveness and manifestations of chronic GVHD after allogeneic stem cell transplantation.

Patients with chronic graft-versus-host disease (cGVHD) have increased B cell-activating factor (BAFF) levels, but whether BAFF promotes disease after allogeneic bone marrow transplantation (allo-BMT) remains unknown. In a major histocompatibility complex-mismatched model with cGVHD-like manifestations, we first examined B-lymphopenic μMT allo-BMT recipients and found that increased BAFF levels in cGVHD mice were not merely a reflection of B-cell number. Mice that later developed cGVHD had significantly increased numbers of recipient fibroblastic reticular cells with higher BAFF transcript levels. Increased BAFF production by donor cells also likely contributed to cGVHD, because BAFF transcript in CD4+ T cells from diseased mice and patients was increased. cGVHD manifestations in mice were associated with high BAFF/B-cell ratios and persistence of B-cell receptor (BCR)-activated B cells in peripheral blood and lesional tissue. By employing BAFF transgenic (Tg) mice donor cells, we addressed whether high BAFF contributed to BCR activation in cGVHD. BAFF increased NOTCH2 expression on B cells, augmenting BCR responsiveness to surrogate antigen and NOTCH ligand. BAFF Tg B cells had significantly increased protein levels of the proximal BCR signaling molecule SYK, and high SYK protein was maintained by BAFF after in vitro BCR activation or when alloantigen was present in vivo. Using T cell-depleted (BM only) BAFF Tg donors, we found that BAFF promoted cGVHD manifestations, circulating GL7+ B cells, and alloantibody production. We demonstrate that pathologic production of BAFF promotes an altered B-cell compartment and augments BCR responsiveness. Our findings compel studies of therapeutic targeting of BAFF and BCR pathways in patients with cGVHD.
Authors
Jia, W; Poe, JC; Su, H; Anand, S; Matsushima, GK; Rathmell, JC; Maillard, I; Radojcic, V; Imai, K; Reyes, NJ; Cardona, DM; Li, Z; Suthers, AN; Curry-Chisolm, IM; DiCioccio, RA; Saban, DR; Chen, BJ; Chao, NJ; Sarantopoulos, S
MLA Citation
Jia, Wei, et al. “BAFF promotes heightened BCR responsiveness and manifestations of chronic GVHD after allogeneic stem cell transplantation.Blood, vol. 137, no. 18, May 2021, pp. 2544–57. Pubmed, doi:10.1182/blood.2020008040.
URI
https://scholars.duke.edu/individual/pub1473570
PMID
33534893
Source
pubmed
Published In
Blood
Volume
137
Published Date
Start Page
2544
End Page
2557
DOI
10.1182/blood.2020008040

An Ear-Tissue Model for High-Resolution In Vivo Imaging.

Authors
Jiao, Y; Zhang, P; DeOliveira, D; Drago, N; Chao, NJ; Chen, BJ
MLA Citation
Jiao, Yiqun, et al. “An Ear-Tissue Model for High-Resolution In Vivo Imaging.Blood, vol. 116, no. 21, AMER SOC HEMATOLOGY, 2010, pp. 623–623.
URI
https://scholars.duke.edu/individual/pub925883
Source
wos
Published In
Blood
Volume
116
Published Date
Start Page
623
End Page
623

Allogeneic Committed Hematopoietic Progenitors Are Protective Against Radiation.

<jats:title>Abstract</jats:title> <jats:p>Whole-body irradiation may lead to bone marrow failure and death. It was previously reported that congenic myeloerythroid-restricted progenitors are able to radioprotect lethally irradiated animals. However, this approach will not be practical because syngeneic/congenic donors are rarely available in humans. To solve this problem, we investigated whether allogeneic committed progenitors are also radioprotective. Hematopoietic committed progenitors were isolated by FACS based on the presence of early progenitor marker CD244 and the absence of stem cell marker CD150 (CD244+CD150−). BALB/c mice (H2d) were lethally irradiated with 8.5 Gy. Within 4 hours of irradiation, the irradiated mice were infused with 5x105 sorted hematopoietic progenitors from major histocompatibility complex mistmatched C57BL/6 donors (H2b). As shown in the Figure B, all the mice in the radiation control group died within 15 days post irradiation (median survival time: 13 days). Infusion of hematopoietic committed progenitors significantly prolonged the survival of the lethally irradiated mice (P=0.0018, median survival time: 28 days). These results are similar to the results obtained from congenic hematopoietic progenitors using 1x105 cells (Figure A, P&lt;0.0001, median survival time: 10 days vs. 28 days). These data suggest that allogeneic hematopoietic committed progenitor cells are also able to mediate radioprotective effects. Similar to the congenic hematopoietic committed progenitors, allogeneic progenitors may also exert radioprotective effects by jumpstarting hematologic recovery post irradiation. These cells may be stockpiled and used as “off-the-shelf” products for radiation injury and other applications.</jats:p> <jats:p>Figure Figure</jats:p>
Authors
Chen, BJ; Deoliveira, D; Chao, NJ
MLA Citation
Chen, Benny J., et al. “Allogeneic Committed Hematopoietic Progenitors Are Protective Against Radiation.Blood, vol. 110, no. 11, American Society of Hematology, 2007, pp. 4871–4871. Crossref, doi:10.1182/blood.v110.11.4871.4871.
URI
https://scholars.duke.edu/individual/pub1382261
Source
crossref
Published In
Blood
Volume
110
Published Date
Start Page
4871
End Page
4871
DOI
10.1182/blood.v110.11.4871.4871

Hematopoiteic stem cell doses correlate with the speed of immune reconstitution after allogeneic stem cell transplantation.

Authors
Chen, BJ; Cui, XY; Chao, NJ
MLA Citation
Chen, B. J., et al. “Hematopoiteic stem cell doses correlate with the speed of immune reconstitution after allogeneic stem cell transplantation.Blood, vol. 96, no. 11, AMER SOC HEMATOLOGY, 2000, pp. 772A-772A.
URI
https://scholars.duke.edu/individual/pub1382273
Source
wos
Published In
Blood
Volume
96
Published Date
Start Page
772A
End Page
772A

Non-GVHD-inducing CD62L− memory T cells promote new T cell regeneration from hematopoietic stem cells

Authors
Chen, BJ; Cui, X; Son, J; Chao, NJ
MLA Citation
Chen, B. J., et al. “Non-GVHD-inducing CD62L− memory T cells promote new T cell regeneration from hematopoietic stem cells.” Biology of Blood and Marrow Transplantation, vol. 10, Elsevier BV, 2004, pp. 23–23. Crossref, doi:10.1016/j.bbmt.2003.12.093.
URI
https://scholars.duke.edu/individual/pub1242645
Source
crossref
Published In
Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation
Volume
10
Published Date
Start Page
23
End Page
23
DOI
10.1016/j.bbmt.2003.12.093

Research Areas:

Administration, Inhalation
Adoptive Transfer
Adult
Airway Resistance
Algorithms
Animals
Anti-Inflammatory Agents, Non-Steroidal
Antineoplastic Agents, Alkylating
Apoptosis
Autoimmune Diseases
B-Lymphocytes
Blood Cell Count
Blood Platelets
Bone Marrow
Bone Marrow Cells
Bone Marrow Purging
Bone Marrow Transplantation
CD4-Positive T-Lymphocytes
Calibration
Cell Count
Cell Death
Cell Differentiation
Cell Division
Cell Separation
Cell Survival
Cell Transplantation
Cells, Cultured
Chemokines
Chimera
Chimerism
Clonal Anergy
Clonal Deletion
Coculture Techniques
Colony-Forming Units Assay
Computer Simulation
Cytokines
DNA
Dendritic Cells
Disease Models, Animal
Diterpenes
Drugs, Chinese Herbal
Ear
Endothelial Cells
Endothelium, Vascular
Epoxy Compounds
Erythrocytes
Escherichia coli
Female
Fetal Blood
Flow Cytometry
Graft Rejection
Graft Survival
Graft vs Host Disease
Graft vs Leukemia Effect
Graft vs Tumor Effect
Growth Hormone
Heart
Hematologic Neoplasms
Hematopoiesis
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Immune System
Immunity
Immunity, Innate
Immunologic Memory
Immunosuppression
Immunosuppressive Agents
Infant
Isoantigens
Kidney
Light
Lipopolysaccharides
Lung
Lymph Nodes
Lymphocyte Activation
Lymphocyte Count
Lymphocyte Depletion
Macrophages, Alveolar
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred MRL lpr
Mice, Knockout
Mice, Transgenic
Models, Animal
Myocardium
Neoplasm Staging
Nephritis
Neutrophil Infiltration
Peptides
Peroxidase
Phantoms, Imaging
Phenanthrenes
Photochemotherapy
Phytotherapy
Pneumonia
Pneumonia, Bacterial
Polymers
Primates
Prodrugs
Pulmonary Alveoli
RNA, Messenger
Radiation Chimera
Radiation Dosage
Radiation Injuries
Radiation Injuries, Experimental
Radiometry
Rats
Receptor, Angiotensin, Type 1
Receptors, Antigen, T-Cell
Recombinant Proteins
Recovery of Function
Reproducibility of Results
Skin
Skin Neoplasms
Skin Transplantation
Specific Pathogen-Free Organisms
Spleen
Stem Cell Transplantation
Stem Cells
Survival Rate
T-Lymphocyte Subsets
T-Lymphocytes
Thymus Gland
Toll-Like Receptor 4
Transplantation Chimera
Transplantation Immunology
Transplantation, Heterologous
Transplantation, Homologous
Tumor Cells, Cultured
Whole-Body Irradiation
Wounds, Penetrating