Animal Pathology

Leadership 

Anne F. Buckley, MD, PhD
919-684-1369
anne.buckley@duke.edu

Location  

RPIV building, Room 112

Resource Overview

The Duke Animal Pathology Core Facility offers experimental pathology support to investigators using animal models to study human disease.

The core facility can provide analysis and interpretation of gross and histologic findings (including blinded review), correlation of pathologic changes with clinical signs and clinical diagnostic results, identification and photography of representative findings for publication, and advice on investigative approaches. Also available is training in animal model analysis techniques including perfusion, necropsy, organ dissection, and tissue preparation. Training in photomicroscopy is available for investigators wishing to do their own data acquisition or analysis, and the core’s imaging and photographic equipment may be operated by qualified users by appointment. The core also provides a referral service, to connect investigators with pathologists in the Duke community who have expertise in particular organ systems.

The facility is directed by a practicing MD PhD pathologist who is board-certified in anatomic pathology and neuropathology, and has extensive basic research training at the graduate and postdoctoral levels with many years of experience in interpretation of animal models of disease. The director’s own research work is in mouse models using in vivo genetic systems analyzed by multiple histological and imaging methods.

The core facility is located in the RPIV building, Room 112. It is equipped with a Leica DM 5000B research microscope with Plan Apo objectives and Differential Interference Contrast, with an automated transmitted light axis for all transmitted light techniques. For image capture, there is an attached Leica DFC 450 (5-megapixel) color digital camera and software with image stitching capabilities.