Michael Cook, PhD
306, 307, 336, 343, and 369 Edwin Jones Cancer Research Building and C313, C316 Levine Sciences Research Center
The Flow Cytometry Shared Resource (FCSR) was established in 1979 to support basic and clinical cancer research.
The FCSR operates, maintains, and upgrades instrumentation for flow cytometic analysis and cell sorting.
Investigators prepare cells and bring them to the FCSR for analysis or cell sorting and pay a fee for the services. These fees are used to partially offset operating expenses.
The FCSR operates four analyzers and four cell sorters, the only ones on campus that are available at all times to all Cancer Center members.
Three of the cell sorters are three laser instruments (one has two lasers) that provide state of the art multiparameter cell sorting of up to 12 simultaneous fluorochromes.
In addition to cell sorting, acquiring, analyzing, archiving, and preparing flow data for publication, the FSCR provides training for self-users of the analyzers, and consultation, technical advice, collaboration, and technical information to potential users.
The FCSR staff has experience in most all areas of flow cytometric analysis and cell sorting applications and strives to help investigators develop new applications. Staff members recommend assays, help to develop and troubleshoot new protocols, participate actively in the data analysis, and, in general, work closely with investigators to fine tune their experiments. An important part of the mission of the FCSR is the education of customers to insure that all users get the most out of the technical resources available. We also participate in the ongoing education of graduate students, post-docs, and MDs.
Another important function is to help investigators evaluate existing cell separation technologies before choosing flow cytometry. Thus, the FCSR staff works to help investigators at all levels of experience and expertise to solve problems, not simply operate flow cytometers.
Although not operated by the Flow Cytometry Shared Resource, there is an image cytometer, located in the LSRC, that combines flow and high speed image analysis in a single platform. For a description of the technology and contact information please follow this link to the Image Stream Core Facility at Duke University:
1. Registration: All potential users of the Cancer Institute Flow Core Facility are required to register for a specific project (fund code) by completing the registration form (see link below). PIs can register multiple projects and users may be registered on more than one project. Once the registration form is completed, email it to the link on the form FacMan@mc.duke.edu, attach it as a PDF and email, or simply print it and hand it to one of our staff. Contact Mike Cook at 613-7818 or email@example.com for self-use training or advice on a new project.
Registration Form (PDF, 341.65 KB)
Once registered, the majority of all scheduling can be done on line using your net ID and net ID password at:
Self users can schedule time using the online calendar for three of the analyzers (FACScan in 316C LSRC-3 colors, and CANTO A or CANTO B-8 colors in 343 Jones Bldg).
Alternatively, customers can make appointments for live cell analysis (FACSCalibur-4 colors or CANTO) by calling 613-7818. Customers can drop off fixed assays with an explanatory key in room 306 Jones Bldg after signing the “Research Signin” clipboard on the counter in room 306. The fridge is under the counter. Evening or weekend dropoffs can be put in CR308, the coldroom just down the hallway from room 306.
Customers can schedule their own sorting times on the DiVa sorter (Lynn Martinek in room 336 Jones, 613-7821) or the FACSVantage sorter (Bin Li in room C313 LSRC, 613-8757) using the online calendar. Typically it takes a couple of sort appointments (via telephone) before a new customer is able to accurately judge how much time to reserve.
Sorting reservations on the MoFlo sorter (Nancy Martin in 336 Jones, 919-613-7836) or the FACStar (Mike in 306 Jones, 919-613-7818) are always made by mutual agreement over the telephone.
When making online sorting reservations, you can utilize the “Experiment Number” field under “New Session” to briefly indicate your parameters (e.g., fluorochromes, number of samples, 96 well plates, etc.)
By using the links below, you can provide the staff with a more detailed description of what you require for your experiment to facilitate proper machine set-up and better utilization of scheduled time.
Request for Analysis (XLS, 16.50 KB)
Request for Sorting (XLS, 17.00 KB)