Michael Cook, PhD
Michael S. Krangel, PhD
The Flow Cytometry Shared Resource (FCSR) was established in 1979 to support investigators conducting basic and clinical cancer research. The FCSR operates, maintains, and upgrades instrumentation for flow cytometric analysis and cell sorting of cells prepared by investigators and brought to the FCSR. The FCSR provides four analyzers, four cell sorters and one image cytometer: these cytometers are available at all times to all DCI members. Two of the cell sorters are four laser instruments, one has three lasers, and one is a two laser sorter. The staff provides state of the art multiparameter cell sorting of up to 12 simultaneous fluorochromes, up to six simultaneous populations, and level P2 biohazard containment. Whether staff-run or self-run, fees are charged to partially offset operating expenses.
In addition to cell sorting, acquiring, analyzing, archiving, and preparing flow data for publication, the FSCR staff provides training of the analyzers and image cytometer for self-users, and consultation, technical advice, collaboration, and technical information for potential users. The analyzers and image cytometer are available 24/7 to trained individuals. The FCSR staff has experience in nearly all areas of flow cytometric analysis and cell sorting applications and strives to help investigators develop new applications. Staff members recommend assays, help to develop and troubleshoot new protocols, participate actively in data analysis, and, in general, work closely with investigators to fine tune their individual experiments. An important part of the mission of the FCSR is the education of customers to insure all users and their lab members get the most out of the technical resources available. We also participate in the ongoing education of graduate students, post-docs, and MDs.
Another important function is to help investigators evaluate existing cell separation technologies before choosing flow cytometry. Thus, the FCSR staff works integrally with investigators at all levels of experience and expertise to solve problems and meet investigators' scientific needs.
All of the cell sorters are multi-laser, have temperature controlled sample and collection options, and have a single cell deposition unit for sorting into multi-well plates.
All potential users of the Cancer Institute Flow Core Facility are required to register for a specific project (fund code) by completing the registration form (see link below). PIs can register multiple projects and users may be registered on more than one project. Once the registration form is completed, email it to the link on the form FacMan@mc.duke.edu, attach it as a PDF and email, or simply print it and hand it to one of our staff. Contact Mike Cook at 613-7818 or email@example.com for self-use training or advice on a new project.
Registration Form (PDF, 341.65 KB)
Once registered, the majority of all scheduling can be done on line using your net ID and net ID password at:
Self users can schedule time using the online calendar for three of the analyzers (FACScan in 316C LSRC-3 colors, and CANTO A or CANTO B in 343 Jones Bldg up to 8 colors, and the Amnis Imagestream in 369 Jones-brightfield and 4 colors). Alternatively, customers can make appointments for live cell analysis (FACSCalibur-4 colors or CANTO) by calling 613-7818. Customers can drop off fixed assays with an explanatory key in room 306 Jones Bldg after signing the "Research Signin" clipboard on the counter in room 306. The fridge is under the counter. Evening or weekend dropoffs can be put in CR308, the coldroom just down the hallway from room 306. Then alert Mike at firstname.lastname@example.org.
Customers can directly schedule their own sorting times on the Astrios sorter (Lynn Martinek in room 336 Jones, 613-7821), the DiVa sorter (Mike Cook in room 307 Jones, 613-7818), or the FACSVantage sorter (Bin Li in room C313 LSRC, 613-8757) using the online calendar. Typically it takes a couple of sort appointments by telephone before a new customer is able to accurately judge how much time to reserve. Sorting reservations on the MoFlo XDP sorter (Nancy Martin in room 336 Jones, 613-7836) are always made by mutual agreement over the telephone.
When making online sorting reservations, you can utilize the "Experiment Number" field under "New Session" with text to briefly indicate your parameters (e.g., fluorochromes, number of samples, 96 well plates, etc.). Cancellations prior to 24 hours before the start of the reservation can easily be made by the client using the online calendar. Cancellations within 24 hours can be made by phone to any of the staff. Please call as soon as you realize that you can't use the time.
By using the links below, you can provide the staff with a more detailed description of what you require for your experiment to facilitate proper machine set-up and better utilization of scheduled time.
Request for Analysis (XLS, 16.50 KB)
Request for Sorting (XLS, 17.00 KB)
Self users are expected to backup their own data utilizing the Shared Resource space on the Medical Center's Isilon server as an intermediary repository. A work station with third party analysis software is available in 369 Jones.
We have only two rates - staff-performed and user-performed - and apply a 40% discount for DCI members to each rate. The standard rate for staff-performed work for non-DCI members is $1.45 per minute (~$87 per hour); that for user-performed work is $0.87 per minute (~$52 per hour). With their added discount, DCI members pay a competitive $0.87 per minute (~$52 per hour) for staff-performed work and $0.52 per minute (~$37 per hour) for user-performed work. Time is recorded in minutes in multiples of 5 minutes. We do not differentiate between cell sorting or analysis, or between data acquisition or analysis. We do not charge for consultation, customer education, or discussion of data with the customer. The work stations are provided to customers free of charge. There is currently no penalty for canceling an appointment if done more than 24 hours before the scheduled start. Late cancellations for staff-performed cell sorting are charged at 50% of the original reserved charge. We do not currently charge for late cancellations on the self-use analyzers.