Fan Yuan

Overview:

Dr. Yuan has extensive experiences in analysis of therapeutic agent transport in mammalian cells, tissues, and organs, and development of effective strategies, design principles, and new technologies that can be used to facilitate the transport. The goal of his research is to improve delivery of therapeutic agents to their targets, which is crucial in treatment and prevention of diseases. He has published >100 scientific papers in peer-reviewed journals, and a textbook on transport analysis in biological systems that has been used to teach undergraduate and graduate courses in many universities.

Positions:

Professor of Biomedical Engineering

Biomedical Engineering
Pratt School of Engineering

Professor in Ophthalmology

Ophthalmology
School of Medicine

Member of the Duke Cancer Institute

Duke Cancer Institute
School of Medicine

Education:

B.S. 1983

Peking University (China)

M.S. 1985

Peking University (China)

Ph.D. 1990

City University of New York

Grants:

University Training Program in Biomolecular and Tissue Engineering

Administered By
Biomedical Engineering
Awarded By
National Institutes of Health
Role
Mentor
Start Date
End Date

University Training Program in Biomolecular and Tissue Engineering

Administered By
Biomedical Engineering
Awarded By
National Institutes of Health
Role
Mentor
Start Date
End Date

Training in Biomolecular and Tissue Engineering

Administered By
Orthopaedic Surgery
Awarded By
National Institutes of Health
Role
Mentor
Start Date
End Date

Upgrade of a Shared Instrumentation Resource in the PSOE: The Laser Scanning Confocal Microscope

Administered By
Biomedical Engineering
Awarded By
Lord Foundation of North Carolina
Role
Co-Principal Investigator
Start Date
End Date

Non-Canonical Pathways for Electrogene Transfer

Administered By
Biomedical Engineering
Awarded By
National Institutes of Health
Role
Principal Investigator
Start Date
End Date

Publications:

A Double-Permeability Poroelasticity Model for Fluid Transport in a Biological Tissue

This work presents a double-permeability poroelasticity model for fluid flows in both the microvascular and interstitial networks in a biological tissue. In the newly developed model, both networks are modeled as porous structures with distinct permeabilities and porosities. The microvascular and the interstitial fluid pressures are hydraulically as well as mechanically coupled together. The numerical results for the steady-state flow in a one-dimensional capillary bed using some preliminary material parameters show that the vascular pressure decreases almost linearly from the arteriole-side to the venule-side. The interstitial fluid pressure (IFP) is elevated by an increase in the venule-side vascular pressure as well as by a decrease in the lymphatic drainage capability. Under a transient flow condition induced by a sudden drop in the venule-side vascular pressure, the IFP may pop up during a very short period of time before decreasing to the reduced steady-state value at long times due to the mechanical coupling between the vascular pressure and IFP which acts much faster than the hydraulic coupling between the two pressures through the vascular walls. Oscillatory mechanical load may produce comparable IFP and promote fluid exchange between the microvessels and interstitium. Finally, a perturbation analysis reveals that a boundary layer for the IFP develops near the tissue boundary. For the first-order approximation, the vascular pressure is decoupled from the IFP and the IFP may be obtained with the first-order vascular pressure as a source.
Authors
Jin, Z; Yuan, F
MLA Citation
Jin, Z., and F. Yuan. “A Double-Permeability Poroelasticity Model for Fluid Transport in a Biological Tissue.” Transport in Porous Media, vol. 147, no. 1, Mar. 2023, pp. 169–95. Scopus, doi:10.1007/s11242-023-01904-w.
URI
https://scholars.duke.edu/individual/pub1565123
Source
scopus
Published In
Transport in Porous Media
Volume
147
Published Date
Start Page
169
End Page
195
DOI
10.1007/s11242-023-01904-w

Physiological Barriers to Drug Transport

Authors
MLA Citation
Yuan, F. “Physiological Barriers to Drug Transport.” Molecular, Cellular, and Tissue Engineering, 2018, pp. 83-1-83–19.
URI
https://scholars.duke.edu/individual/pub1551014
Source
scopus
Published Date
Start Page
83-1-83-19

Delivery of plasmid DNA through intratumoral infusion and electroporation

We investigated DNA transport in the interstitial spaceand across cell membrane facilitated by intratumoral infusionand in vivo electroporation, respectively. In the study, a ratfibrosarcoma was perfused ex vivo, and apparent hydraulicconductivity (Kaap) was quantified under different perfusionconditions. In addition, three plasmid DNA vectors wereinfused into solid tumors. Immediately after infusion, tumorswere treated with or without electric pulses. Gene expressionand tumor growth delay were determined at different timepoints after electroporation. We found that K m was verysensitive to the perfusion pressure, presumably due toperfusion-induced tissue deformation. Treatment of tumorswith electric pulse facilitated gene expression in vivo. Thegrowth of tumors treated with plasmid DNA encodinginterleukin 12 (IL-12) and electric pulses was slower thanthose treated with IL-12 or electric pulses alone. These datasuggest that gene delivery in solid tumors could be improvedsignificantly through combination of intratumoral infusion andin vivo electroporation.
Authors
Yuan, F; Zaharoff, D; Zhang, XY; Lohr, F; Dewhirst, MW; Li, CY
MLA Citation
Yuan, F., et al. “Delivery of plasmid DNA through intratumoral infusion and electroporation.” Asme International Mechanical Engineering Congress and Exposition, Proceedings (Imece), vol. 2000-F, 2000, pp. 125–28. Scopus, doi:10.1115/IMECE2000-2231.
URI
https://scholars.duke.edu/individual/pub1502506
Source
scopus
Published In
Asme International Mechanical Engineering Congress and Exposition, Proceedings (Imece)
Volume
2000-F
Published Date
Start Page
125
End Page
128
DOI
10.1115/IMECE2000-2231

An Enhanced Tilted-Angle Acoustofluidic Chip for Cancer Cell Manipulation

In recent years, surface acoustic wave (SAW) devices have demonstrated great potentials and increasing applications in the manipulation of nano- and micro-particles including biological cells with the advantages of label-free, high sensitivity and accuracy. In this letter, we introduce a novel tilted-angle SAW devices to optimise the acoustic pressure inside a microchannel for cancer-cell manipulation. The SAW generation and acoustic radiation force are improved by seamlessly patterning electrodes in the space surrounding the microchannel. Comparisons between this novel SAW device and a conventional device show a 32% enhanced separation efficiency while the input power, manufacturing cost and fabrication effort remain the same. Effective separation of HeLa cancer cells from peripheral blood mononuclear cells is demonstrated. This novel SAW device has the advantages in minimizing device power consumption, lowering component footprint and increasing device density.
Authors
Wu, F; Shen, MH; Yang, J; Wang, H; Mikhaylov, R; Clayton, A; Qin, X; Sun, C; Xie, Z; Cai, M; Wei, J; Liang, D; Yuan, F; Wu, Z; Fu, Y; Yang, Z; Sun, X; Tian, L; Yang, X
MLA Citation
Wu, F., et al. “An Enhanced Tilted-Angle Acoustofluidic Chip for Cancer Cell Manipulation.” Ieee Electron Device Letters, vol. 42, no. 4, Apr. 2021, pp. 577–80. Scopus, doi:10.1109/LED.2021.3062292.
URI
https://scholars.duke.edu/individual/pub1477216
Source
scopus
Published In
Ieee Electron Device Letters
Volume
42
Published Date
Start Page
577
End Page
580
DOI
10.1109/LED.2021.3062292

Correction: Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB.

Correction for 'Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB' by Roman Mikhaylov et al., Lab Chip, 2020, 20, 1807-1814, DOI: 10.1039/C9LC01192G.
Authors
Mikhaylov, R; Wu, F; Wang, H; Clayton, A; Sun, C; Xie, Z; Liang, D; Dong, Y; Yuan, F; Moschou, D; Wu, Z; Shen, MH; Yang, J; Fu, Y; Yang, Z; Burton, C; Errington, RJ; Wiltshire, M; Yang, X
MLA Citation
Mikhaylov, Roman, et al. “Correction: Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB.Lab on a Chip, vol. 20, no. 17, Aug. 2020, p. 3278. Epmc, doi:10.1039/d0lc90070b.
URI
https://scholars.duke.edu/individual/pub1453844
PMID
32735307
Source
epmc
Published In
Lab on a Chip
Volume
20
Published Date
Start Page
3278
DOI
10.1039/d0lc90070b