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Amy Gladfelter

Instructor in the Department of Cell Biology

Amy Gladfelter

Positions

Instructor in the Department of Cell Biology in the School of Medicine

2023 School of Medicine

Member of the Duke Cancer Institute in the School of Medicine

2023 School of Medicine

Education

Ph.D. 2001

2001 Duke University

Publications, Grants & Awards

Grants (7)
Academics Articles (111)
Book Sections (2)
Conference Pages (2)

The role for phase separation in oncogenesis and aberrant chromatin looping formation

2023-2027 National Institutes of Health

Biophysics of the nucleus

2023-2026 Massachusetts Institute of Technology

The role of biomolecular condensates in regulating the cytoskeleton

2023-2025 National Institutes of Health

Double-stranded RNA dictates SARS-CoV-2 nucleocapsid condensation temperature

2023-2025 National Institutes of Health

Dissecting the mechanistic role of multinucleation in breast and trophoblast cancers

2023-2024 National Institutes of Health

Gene Expression Variability and Nuclear Territory Autonomy in Syncytia

2023-2024 National Institute of General Medical Sciences

Cytoplasmic Organization by phase transitions

2023-2024 National Institutes of Health

Dynamical control enables the formation of demixed biomolecular condensates.

November 24, 2023 Nature Communications

Malassezia is widespread and has undescribed diversity in the marine environment

October 01, 2023 Fungal Ecology

Live-cell imaging of septins and cell polarity proteins in the growing dikaryotic vegetative hypha of the model mushroom Coprinopsis cinerea.

June 22, 2023 Scientific Reports

A gene duplication of a septin reveals a developmentally regulated filament length control mechanism.

March 06, 2023 The Journal of Cell Biology

RNA structure controls composition and network dynamics of condensates

February 10, 2023 Biophysical Journal

The role of RNA condensation in reducing gene expression noise

February 10, 2023 Biophysical Journal

Curvature sensing as an emergent property of multiscale assembly of septins.

February 07, 2023 Proc Natl Acad Sci U S A

Targeted secretion: Myosin V delivers vesicles through formin condensates.

November 07, 2022 Curr Biol

Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures.

August 12, 2022 Nucleic Acids Res

PRD-2 mediates clock-regulated perinuclear localization of clock gene RNAs within the circadian cycle of Neurospora.

August 02, 2022 Proc Natl Acad Sci U S A

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions.

July 28, 2022 Journal of Visualized Experiments : Jove

Role of spatial patterning of N-protein interactions in SARS-CoV-2 genome packaging.

April 19, 2022 Biophysical Journal

Membrane surfaces regulate assembly of ribonucleoprotein condensates.

April 01, 2022 Nat Cell Biol

Dilute phase oligomerization can oppose phase separation and modulate material properties of a ribonucleoprotein condensate.

March 29, 2022 Proc Natl Acad Sci U S A

Design considerations for analyzing protein translation regulation by condensates.

January 01, 2022 Rna

20 years of Developmental Cell: Looking forward.

December 06, 2021 Dev Cell

Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa.

November 01, 2021 Fungal Genet Biol

Evolution and Physiology of Amphibious Yeasts.

October 08, 2021

Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling.

October 01, 2021 Molecular Biology of the Cell

Getting droplets into shape.

September 10, 2021 Science

Role of spatial patterning of N-protein interactions in SARS-CoV-2 genome packaging.

July 20, 2021 Biophysical Journal

Roadmap for the multiscale coupling of biochemical and mechanical signals during development.

April 14, 2021 Physical Biology

Culturing and Multiplexed Time-Lapse Imaging of Fungal Isolates from Marine and Coastal Environments.

April 01, 2021 Curr Protoc

Moving beyond disease to function: Physiological roles for polyglutamine-rich sequences in cell decisions.

April 01, 2021 Curr Opin Cell Biol

RNA contributions to the form and function of biomolecular condensates.

March 01, 2021 Nat Rev Mol Cell Biol

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.

February 05, 2021 Science

The state of the septin cytoskeleton from assembly to function.

February 01, 2021 Curr Opin Cell Biol

Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid.

December 17, 2020 Mol Cell

Shed light in the dark lineages of the fungal tree of lifeâstres

December 01, 2020 Life (Basel)

Modeling the Mechanisms by Which Coexisting Biomolecular RNA-Protein Condensates Form.

November 24, 2020 Bull Math Biol

The hierarchical assembly of septins revealed by high-speed AFM.

October 08, 2020 Nature Communications

Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity.

July 06, 2020 The Journal of Cell Biology

Spatial heterogeneity of the cytosol revealed by machine learning-based 3D particle tracking.

July 01, 2020 Molecular Biology of the Cell

FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells.

April 06, 2020 The Journal of Cell Biology

Guanidine hydrochloride reactivates an ancient septin hetero-oligomer assembly pathway in budding yeast.

January 28, 2020 Elife

Unconventional Cell Division Cycles from Marine-Derived Yeasts.

October 21, 2019 Curr Biol

The Control Centers of Biomolecular Phase Separation: How Membrane Surfaces, PTMs, and Active Processes Regulate Condensation.

October 17, 2019 Mol Cell

An amphipathic helix enables septins to sense micrometer-scale membrane curvature.

April 01, 2019 The Journal of Cell Biology

Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.

March 20, 2019 Neuron

Marine fungi.

March 18, 2019 Curr Biol

Fungi in the Marine Environment: Open Questions and Unsolved Problems.

March 05, 2019 Mbio

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates.

January 24, 2019 Cell

Partial demixing of RNA-protein complexes leads to intradroplet patterning in phase-separated biological condensates.

January 01, 2019 Phys Rev E

A New Lens for RNA Localization: Liquid-Liquid Phase Separation.

September 08, 2018

Principles of Systems Biology, No. 30.

July 25, 2018 Cell Systems

mRNA structure determines specificity of a polyQ-driven phase separation.

May 25, 2018 Science

LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching.

May 07, 2018 The Journal of Cell Biology

Control of septin filament flexibility and bundling by subunit composition and nucleotide interactions.

March 15, 2018 Molecular Biology of the Cell

The Unsolved Problem of How Cells Sense Micron-Scale Curvature.

December 01, 2017 Trends in Biochemical Sciences

Lessons from Yeast on How to Avoid Stress Eating.

October 09, 2017 Dev Cell

What your PI forgot to tell you: why you actually might want a job running a research lab.

July 01, 2017 Molecular Biology of the Cell

Non-model model organisms.

June 29, 2017 Bmc Biology

Analysis of septin reorganization at cytokinesis using polarized fluorescence microscopy

May 03, 2017 Frontiers in Cell and Developmental Biology

Septin complexes assemble during a kinetic window of opportunity.

November 01, 2016 Cell Cycle

Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells.

October 18, 2016 Proc Natl Acad Sci U S A

Clustered nuclei maintain autonomy and nucleocytoplasmic ratio control in a syncytium.

July 01, 2016 Molecular Biology of the Cell

Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton.

April 11, 2016 The Journal of Cell Biology

Quantitative spatial analysis of transcripts in multinucleate cells using single-molecule FISH.

April 01, 2016 Methods

In vitro reconstitution of septin assemblies on supported lipid bilayers.

January 01, 2016 Methods in Cell Biology

Nuclear autonomy in multinucleate fungi.

December 01, 2015 Curr Opin Microbiol

How nontraditional model systems can save us.

November 01, 2015 Molecular Biology of the Cell

RNA Controls PolyQ Protein Phase Transitions.

October 15, 2015 Mol Cell

Editorial overview: host-microbe interactions: fungi: heterogeneity in fungal cells, populations, and communities.

August 01, 2015 Curr Opin Microbiol

Life as a moving fluid: fate of cytoplasmic macromolecules in dynamic fungal syncytia.

August 01, 2015 Curr Opin Microbiol

Septin Form and Function at the Cell Cortex.

July 10, 2015 The Journal of Biological Chemistry

Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.

June 10, 2015 Nano Letters

Polarized Fluorescence Microscopy to Study Cytoskeleton Assembly and Organization in Live Cells.

June 01, 2015 Curr Protoc Cell Biol

Cytoskeletal dynamics: a view from the membrane.

May 11, 2015 The Journal of Cell Biology

Biological Significance of Photoreceptor Photocycle Length: VIVID Photocycle Governs the Dynamic VIVID-White Collar Complex Pool Mediating Photo-adaptation and Response to Changes in Light Intensity.

May 01, 2015 Plos Genet

MultiFocus Polarization Microscope (MF-PolScope) for 3D polarization imaging of up to 25 focal planes simultaneously.

March 23, 2015 Optics Express

Ploidy variation in multinucleate cells changes under stress.

March 15, 2015 Molecular Biology of the Cell

PolyQ-dependent RNA-protein assemblies control symmetry breaking.

March 02, 2015 The Journal of Cell Biology

Septins and Generation of Asymmetries in Fungal Cells.

January 01, 2015

Fungal pathogens are platforms for discovering novel and conserved septin properties.

August 01, 2014 Curr Opin Microbiol

Cytoskeleton: cirque du septins.

June 02, 2014 Curr Biol

Septin assemblies form by diffusion-driven annealing on membranes.

February 11, 2014 Proc Natl Acad Sci U S A

Nuclear repulsion enables division autonomy in a single cytoplasm.

October 21, 2013 Curr Biol

Protein aggregation behavior regulates cyclin transcript localization and cell-cycle control.

June 24, 2013 Dev Cell

Septin phosphorylation and coiled-coil domains function in cell and septin ring morphology in the filamentous fungus Ashbya gossypii.

February 01, 2013 Eukaryot Cell

Septin ring size scaling and dynamics require the coiled-coil region of Shs1p.

September 01, 2012 Molecular Biology of the Cell

Heterogeneity in mitochondrial morphology and membrane potential is independent of the nuclear division cycle in multinucleate fungal cells.

March 01, 2012 Eukaryot Cell

Spatial guidance of cell asymmetry: septin GTPases show the way.

February 01, 2012 Traffic

Axl2 integrates polarity establishment, maintenance, and environmental stress response in the filamentous fungus Ashbya gossypii.

December 01, 2011 Eukaryot Cell

Rapid and quantitative imaging of excitation polarized fluorescence reveals ordered septin dynamics in live yeast.

August 17, 2011 Biophysical Journal

Septin filaments exhibit a dynamic, paired organization that is conserved from yeast to mammals.

June 13, 2011 The Journal of Cell Biology

Guides to the final frontier of the cytoskeleton: septins in filamentous fungi.

December 01, 2010 Curr Opin Microbiol

Physical interaction between VIVID and white collar complex regulates photoadaptation in Neurospora.

September 21, 2010 Proc Natl Acad Sci U S A

A conserved Gâ regulatory circuit promotes asynchronous behavior of nuclei sharing a common cytoplasm.

September 15, 2010 Cell Cycle

Stuck in a checkpoint: A role for Ras deregulation in DNA damage checkpoint recovery

September 01, 2010 Cell Cycle

Cellular requirements for the small molecule forchlorfenuron to stabilize the septin cytoskeleton.

June 01, 2010 Cytoskeleton (Hoboken)

Dancing genomes: fungal nuclear positioning.

December 01, 2009 Nat Rev Microbiol

Regulation of distinct septin rings in a single cell by Elm1p and Gin4p kinases.

April 01, 2009 Molecular Biology of the Cell

Pathocycles.

January 01, 2008 Fungal Genetics and Biology : Fg & B

Limited functional redundancy and oscillation of cyclins in multinucleated Ashbya gossypii fungal cells.

March 01, 2007 Eukaryotic Cell

The anaphase-promoting complex/cyclosome is required for anaphase progression in multinucleated Ashbya gossypii cells.

February 01, 2007 Eukaryotic Cell

Nuclear anarchy: asynchronous mitosis in multinucleated fungal hyphae.

December 01, 2006 Current Opinion in Microbiology

AgSwe1p regulates mitosis in response to morphogenesis and nutrients in multinucleated Ashbya gossypii cells.

October 01, 2006 Molecular Biology of the Cell

Erratum: Control of filamentous fungal cell shape by septins and formins (Nature Reviews Microbiology (2006) vol. 4 (223-229) 10.1038/nrmicro.1345)

April 01, 2006 Nature Reviews. Microbiology

Control of filamentous fungal cell shape by septins and formins.

March 01, 2006 Nature Reviews. Microbiology

Asynchronous nuclear division cycles in multinucleated cells.

January 30, 2006 The Journal of Cell Biology

Interplay between septin organization, cell cycle and cell shape in yeast.

April 15, 2005 Journal of Cell Science

Genetic interactions among regulators of septin organization.

August 01, 2004 Eukaryotic Cell

Cdc42p, GTP hydrolysis, and the cell's sense of direction.

July 01, 2004 Cell Cycle

Scaffold-mediated symmetry breaking by Cdc42p.

December 01, 2003 Nature Cell Biology

Septin ring assembly involves cycles of GTP loading and hydrolysis by Cdc42p.

January 21, 2002 The Journal of Cell Biology

A lab with a view: American postdocs abroad.

January 01, 2002 Cell Biology Education

The septin cortex at the yeast mother-bud neck.

December 01, 2001 Current Opinion in Microbiology

Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud.

November 12, 2001 The Journal of Cell Biology

Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud

October 29, 2001 The Journal of Cell Biology

Isolation and characterization of effector-loop mutants of CDC42 in yeast.

May 01, 2001 Molecular Biology of the Cell

Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae.

October 01, 2000 Molecular and Cellular Biology

Probing RNA Structure in Liquid-Liquid Phase Separation Using SHAPE-MaP.

2018

Septins in Four Model Fungal Systems: Diversity in Form and Function

2008 The Septins

Identifying fungal regulatory motif patterns using SCOPE, an ensemble learning method motif finder

2007 Proceedings of the 7th IEEE International Conference on Bioinformatics and Bioengineering, BIBE

Seeking truth on Monte Verita. Workshop on the molecular biology and biochemistry of septins and septin function.

2007 Embo Reports

amy.gladfelter@duke.edu

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Cancer Types
Primary Brain Cancer
Brain and Spine Metastasis
Breast Cancer
Endocrine Neoplasia
Gastrointestinal Cancer
Gynecologic Cancer
Head and Neck Cancer
Hematologic Malignancies and Cellular Therapy
Melanoma and Advanced Skin Cancers
Pediatric Cancers
Prostate and Urologic Cancers
Sarcoma
Thoracic Cancer
Cancer Therapies
Immunotherapy
Molecular Tumor Board
Supportive Care
Supportive Care and Survivorship Center
Research
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