Jack Keene

James B. Duke Distinguished Professor of Molecular Genetics and Microbiology
Jack Keene
Jack Keene

Overview

The Keene Laboratory has a long-term interest in the structure and function of viral and mammalian genomes. Having determined the first genomic sequences for rabies, Ebola, Marburg and vesicular stomatitis virus, and discerned the origins of defective interfering viruses, interests shifted to the cloning of six human genes involved in autoimmune reactivity. This resulted in the identification of numerous autoimmune RRM-type RNA-binding proteins the discovery of the RRM, and the RNA targets to which they bind. The current interests of the lab surround the functions of the human RRM-ELAV/Hu proteins that are bound to a subset of cellular mRNAs involved in growth regulation neuronal plasticiyt and cancer. The laboratory demonstrated that ELAV/Hu proteins bind and regulate the expression of early response gene transcripts such as those encoding the protooncogene and cytokine proteins.

In addition, it was shown that while stabilizing these mRNAs and/or activating their translation, the ELAV/Hu proteins participate in cellular , differentiation and carcinogenesis. More recently, the laboratory has examined dozens of RNA-binding proteins in order to identify large numbers of structurally
and/or functionally related mRNAs that cluster in vivo based upon their binding to these proteins. This has been termed ribonomics because it involves parallel analysis of mRNA subsets en masse based upon their presence in messenger ribonucleoprotein complexes. This new approach to functional genomics is being applied to virus-infected cells, tumors and cells treated with various agents. Ribonomics has led to the identification of mRNA clusters that are posttranscriptionally regulated, and represent the organizational state of genetic information between the genome and the proteome. Dr. Keen has propsed the existence of post-transcriptional operons based upon the association of structurally and functionally-linked mRNAs in vivo.

Positions

James B. Duke Distinguished Professor of Molecular Genetics and Microbiology in the School of Medicine

1997 School of Medicine

Professor of Molecular Genetics and Microbiology in the School of Medicine

1989 School of Medicine

Associate Professor in Medicine in the School of Medicine

2020 School of Medicine

Member of the Duke Cancer Institute in the School of Medicine

1979 School of Medicine

Education

Ph.D. 1974

1974 University of Washington

Publications, Grants & Awards

Offices & Contact

207 Research Drive, 414 Jones Box 3020
Durham, NC
27717
Duke Box 3020
Durham, NC
27710