Overview

Established in 1996, the Molecular Genomics Core (MGC) is a state-of-the-art multiomics facility equipped with high-sensitivity experimental platforms, enabling tailoring of experiments to meet researcher's genetic, genomic, epigenomic and proteomic needs. They work diligently to facilitate the success of your project from planning, data generation, and analysis.

The Molecular Genomics Core highlights applications that include:

• 10x Genomics single-cell library (Chromium) for gene expression, immune profiling, and ATAC sequencing
• Transcriptomic spatial gene expression (Visium) and in situ sequencing (Xenium) platforms
• Taqman targeted SNP genotyping
• Quanterix Simoa bead-based immunoassay technology, capable of detecting proteins at femtogram levels in serum and plasma

Services and Technologies

10x Genomics Single-cell Solutions

• 3’ and 5’ gene expression (snRNAseq & scRNAseq)
• 5’ V(D)J profiling in T and B cells
• ATAC-seq
• Cell surface protein expression
• Cell multiplexing
• Flex (Fixed RNA profiling)

Spatial Profiling

• Visium Spatial Gene Expression profiling (whole transcriptome)
• Xenium in Situ Gene Expression profiling (targeted)

SNP Genotyping

• TaqMan SNP Genotyping Assays

Digital Immunoassay Biomarker Detection

• Quanterix Simoa HD-X instrument

Sample Quantitation and Quality Assessment

• Qubit 3.0 Fluorometer
• Agilent 4200 TapeStation System

Equipment

10X Genomics Chromium Controller & Chromium X

• Unique reagent delivery system that partitions individual cells into 100,000s to 1,000,000s of uniquely addressable partitions.

10x Genomics Visium CytAssist

• Facilitates transfer of analytes from glass slide onto Visium slide.

10x Genomics Xenium

• Newly released in Situ Gene Expression platform with human, mouse, and custom gene panels.

Quanterix HD-X Analyzer

• Simoa bead-based immunoassay technology by Quanterix provides 1000x greater sensitivity than traditional immunoassays.
• Biomarkers measure at fg/ml concentrations in serum, plasma, and CSF.

ThermoFisher QuantStudio 5 Real-Time PCR System

Sample Quantitation and Quality Assessment Instruments

• Agilent 4200 TapeStation
• Thermo Scientific Qubit 3.0 Fluorometer
• Tecan Infinite M200Pro Scanner
• Nexcelom Cellometer Ascend Fluorescent Viability Cell Counter
• Nexcelom Cellometer K2 Fluorescent Viability Cell Counter

Sample Handling Instruments

• BioMicroLab XL-20 Tube Sorter
• Integra VIAFLO 96 electronic pipetting system

Contacts

Please email the team at dmpi.mgc@duke.edu.

• Simon Gregory, PhD, Director
• Karen Abramson, Assistant Director
• Emily Hocke, Research Analyst
• Stephanie Arvai, Research Analyst
• Vaibhav Jain, Bioinformatician, Analysis Team Lead

Location
DMPI
Carmichael Building, Lab 51-118
300 North Duke Street, Durham, NC