Established in 1996, the Molecular Genomics Core is a state-of-the-art facility offering a variety of experimental platforms to facilitate (epi)genomics and proteomics research. Accredited as a Duke Core facility in 2007, the MGC offers decades of experience with genetic, genomic, epigenomic, and proteomic study design and technology, and works closely with researchers to customize experiments to meet their needs.

The Molecular Genomics Core highlights applications that include:

  • 10x Genomics single-cell library (Chromium) for both gene expression and ATAC sequencing
  • spatial gene expression (Visium) and in Situ gene expression (Xenium) platforms
  • DNA methylation microarrays
  • SNP genotyping and copy number microarrays
  • Taqman targeted SNP genotyping
  • Quanterix Simoa® bead-based immunoassay technology is capable of detecting proteins at femtogram levels in serum and plasma.

Learn more about the state-of-the-art Xenium platform.

Services and Technologies

10x Genomics Single-cell Solutions

  • Gene expression (snRNAseq & scRNAseq)
  • V(D)J profiling in T and B cells
  • ATAC-seq
  • Cell surface protein expression
  • Cell Multiplexing
  • Flex (Fixed RNA profiling)

Spatial Profiling

  • Visium Spatial Gene Expression profiling  (whole transcriptome)
  • Xenium in Situ Gene Expression profiling (targeted)
  • Akoya Phenocycler for spatial protein profiling

DNA Methylation

  • Genome-wide:  Illumina methylation EPIC Arrays
  • SNP Genotyping and Copy Number Variation
  • Genome-wide:  Illumina microarrays
  • Targeted:  Illumina microarrays and Life Technologies Taqman Assays

Sample Quantitation and Quality Assessment

Whole pup run on mouse tissue atlassing panel
Image provided 10x Genomics – Xenium Mouse Multi-Tissue Atlassing Panel


10X Genomics Chromium Controller & Chromium X

  • Unique reagent delivery system that partitions individual cells into 100,000s to 1,000,000s of uniquely addressable partitions.

10x Genomics Visium CytAssist

  • Facilitates transfer of analytes from glass slide onto Visium slide.

10x Genomics Xenium

  • Newly released in Situ Gene Expression platform with human, mouse, and custom gene panels.

Akoya Phenocycler with Zeiss microscope

  • Fluidics and microscopic imaging system that allows for multiplexed profiling of up to 100 proteins at true single-cell resolution.

Illumina iScan System and Autoloader 2

  • High-precision, high-throughput array scanning for Illumina’s expansive portfolio of genetic analysis assays.

Quanterix HD-X Analyzer

  • Simoa bead-based immunoassay technology by Quanterix provides 1000x greater sensitivity than traditional immunoassays.
  • Biomarkers measure at fg/ml concentrations in serum, plasma, and CSF.

ThermoFisher QuantStudio 5 Real-Time PCR System

Sample Quantitation and Quality Assessment Instruments

  • Agilent 4200 TapeStation
  • Thermo Scientific Qubit 3.0 Fluorometer
  • Tecan Infinite M200Pro Scanner
  • Nexcelom Cellometer K2 Fluorescent Viability Cell Counter

Sample Handling Instruments

  • Tecan Freedom Evo 150 Robotic Liquid Handling System
  • BioMicroLab XL-20 Tube Sorter
  • Integra VIAFLO 96 electronic pipetting system


  • Simon Gregory, PhD, Director
  • Karen Abramson, Assistant Director
  • Emily Hocke, Research Analyst
  • Stephanie Arvai, Research Analyst

Carmichael Building, Lab 51-118
300 North Duke Street, Durham, NC 

This page was reviewed on 09/18/2023